Analysis of Circulating Pulmonary Endothelial Cells
Benjamin Gaston
Primary Investigator
Brief description of study
What is the purpose of this study?
Endothelial cells line blood vessels to maintain vascular integrity and permeability. When these cells become damaged, they are shed from the lining of the blood vessels into the bloodstream and become known as circulating endothelial cells (CECs). The purpose of this research is to study circulating endothelial cells (CECs) in subjects with pulmonary arterial hypertension (PAH) and compare those findings with CECs from persons who don’t have PAH, sometimes referred to as healthy controls.
By studying the differences of CECs in subjects with and without PAH, we hope to understand how these influence PAH and PAH disease progression.
Detailed description of study
What will happen during the study?
Attend one study visit that will last about 2 hours (study procedures will take place at Indiana University Health (University Hospital):
- During this visit, you will complete a 6-minute walk test (6MWT) while wearing an airway clearance vest, followed immediately by activating the vest and drawing 40mL of arterial blood.
- We will take your vital signs before the 6MWT and again after the blood draw.
- We will also measure your body’s S-nitrosothiol (SNO) level using a nitrous oxide analyzer (NOA) before and after the 6MWT.
Incentive/compensation
- You will receive $50 in the form of a payment card for completing the study requirements.
Eligibility of study
You may be eligible for this study if you meet the following criteria:
- Conditions: Healthy, Pulmonary arterial hypertension, PAH
-
Age: 18 years - 60 years
-
Gender: All
Inclusion criteria:
- Have either been diagnosed with PAH or are a healthy control (do not have PAH)
Exclusion criteria:
PA pressure over 2/3 systemic
Presence of unrepaired cyanotic congenital heart disease or presence of hemodynamically significant intracardiac shunt
Anemia
Recent history of syncope (prior 6 months)
Pregnant
Inflammatory lung disease – either intercurrent or causing the PAH
Hematological disorder, including polycythemia or neoplastic disease