Evaluation Of Endothelial Cell And Other Lung Cell Populations In Lung Explants From Pvd Patients
Investigating Lung Cell Populations in Pulmonary Vascular Disease
David Roe, MD
Primary Investigator
Brief description of study
For patients with pulmonary vascular disease (PVD) in its end stage, lung transplantation becomes the only viable treatment option. Our study proposes to utilize lung explants from transplant recipients, organs deemed unsuitable/rejected for transplantation, and donors to evaluate cellular populations and molecular mechanisms in both diseased and healthy conditions, offering insights that are difficult to obtain through other means. By comparing the cellular composition, gene expression profiles, and structural changes in these tissues, we aim to elucidate the pathophysiological processes underlying these severe pulmonary conditions.
Detailed description of study
2.1 Primary objective: Identify populations of pulmonary vascular endothelial cells in explant lungs from pulmonary vascular disease patients.
2.2 Secondary objective: Identify differences between patients with pulmonary vascular diseases and controls (donors who consented for research and/or rejected transplants) regarding their pulmonary vascular endothelial cell populations.
DONOR
- Time of Transplant (recipient): tissue harvest by surgical staff – overseen by the Research Coordinator
- Lung explant - leftover diseased lung tissue from the recipient, not needed by the pathologist, will be added to a conical tube containing culture media (DMEM Gibco) and into a formalin solution for the histological examination. The tissues will be handled by research personnel to pick up right after the surgery.
RECIPIENT
- Potential lung transplant recipients from Methodist Hospital (Indianapolis, IN) will be enrolled.
- Time of Transplant (donor): tissue harvest by surgical staff-overseen by the Research Coordinator
- Lung explant – leftover lung tissue from the donor, not needed by the surgical staff for the clinically authorized surgical procedure will be added to a conical tube containing culture media (DMEM Gibco) and into a formalin solution for the histological examination. The tissues will be handled by research personnel to pick up right after the surgery.
Eligibility of study
You may be eligible for this study if you meet the following criteria:
- Conditions: Pulmonary vascular disease (PVD), Healthy
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Age: 18 years - 60 years
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Gender: All
Eligibility Criteria:
- Ages 18-60 years old
- Diagnosis of PVD or Healthy
- English-speaking
This study investigates lung cell populations in patients with pulmonary vascular disease (PVD). Pulmonary vascular disease refers to conditions affecting the blood vessels in the lungs, which can lead to serious health issues. The purpose of this study is to understand the differences in lung cell populations between those with PVD and healthy individuals by analyzing lung tissue samples.
Participants in this study will include those undergoing lung transplants and healthy individuals. Lung tissue samples will be collected during transplant procedures. These samples will be analyzed to study the cellular makeup and gene expression profiles, which will help understand the disease better. The samples will be placed in culture media and formalin for examination.
- Who can participate: Participants must be between 18 and 60 years old and either have a diagnosis of pulmonary vascular disease or be healthy. They must be able to speak English.
- Study details: Participants will have lung tissue samples collected during transplant procedures. These samples will be analyzed to study the differences in lung cell populations.
